Journal: Biophysical Journal

Article Title: Visualizing the Analogy between Competitive Adsorption and Colloid Stability to Restore Lung Surfactant Function

doi: 10.1016/j.bpj.2012.01.014

Figure Lengend Snippet: Axial fluorescence intensity profiles measured via confocal microscopy. (A) Solvent-spread DPPC monolayer at the air-water interface representing a δ-function in fluorescence intensity used to measure the microscope PSF. The solid lines in (B–D) represent the convolution of the proposed concentration profiles (dotted lines) with the PSF experimentally measured in (A). (B) Fluorescence intensity profile of Survanta on a buffered-saline subphase. (C) Fluorescence intensity profile of albumin in a buffered-saline subphase. (D) Fluorescence intensity profile of either chitosan or PEG in a buffered-saline subphase. Profiles were calculated from z-stacks acquired at a surface pressure of 35 mN/m (B) or a fixed trough surface area of 115 cm2 (C and D). Negative axial locations represent the air, positive locations correspond to the aqueous subphase, and the air-liquid interface is at zero.

Article Snippet: To form the monolayer, 20 μ g of DPPC (Avanti Polar Lipids, Alabaster, AL) doped with 0.5 wt% TR-DPPE in a 2 mg/mL chloroform solution were spread dropwise onto a subphase of 120 mL of ultrapure water at 25°C.

Techniques: Fluorescence, Confocal Microscopy, Microscopy, Concentration Assay

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Emodin was found to inhibit DPP4 activity after screening a natural compound library.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Activity Assay, Drug discovery

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Anthraquinone compounds inhibit DPP4 activity.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Activity Assay, Inhibition, Binding Assay

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Docking model reveals the binding mode of emodin to DPP4 protein.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Binding Assay

Journal: PeerJ

Article Title: Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4

doi: 10.7717/peerj.3283

Figure Lengend Snippet: Emodin treatment (3, 10 and 30 mg/kg, P.O.) in mice decreased the plasma DPP4 activity in a dose-dependent manner.

Article Snippet: The DPP4 screening assay was conducted using a DPP4 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s protocol.

Techniques: Clinical Proteomics, Activity Assay